Does anyone have a protocol for conjugation of an ester dye to gelatin?

More Barbara Szynal's questions See All

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Can formaldehyd-fixed gelatin be melt?

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Could dyes amplify the spectrum of light to a specific wavelength?

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Concentration of gelatin as brain model ?

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Waht is the effect of isoelectric point on MB dye photodegradation by ZnO nanoparticles?

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What is the exact procedure and materials for converting from ester to carbolic acid by base hydrolysis??

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Sybergreen qpcr troublshoulting?

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Adam B Shapiro

Here is a paper that includes preparation of fluorescent gelatin.

Article Fluorescence labeling of gelatin and methylcellulose: Monito...

You can also buy it.

https://www.thermofisher.com/order/catalog/product/G13186

https://www.biovision.com/gelatin-fluorescein-fitc-conjugate-16274.html

Michael G. Weller

All standard protocols should work. Usually, antibodies are to be labeled and not gelatin. This is the reason why gelatin (and other proteins and amine-containing compounds) should be avoided. Suitable buffers are borate, carbonate, or phosphate at a pH of around 8.

Adeyinka Aina

Check the following link:

Article Injectable oligomer-cross-linked gelatine hydrogels via anhy...