Does anyone have a method of creating gene knockouts from Mycobacterium tuberculosis that has successful worked?

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Soumajit Mukherjee

Hi Johannes,

Not an expert in this field, but this article is from one of my colleague's lab and the work was quite well-appreciated. Take a look.

Gene silencing by CRISPR interference in mycobacteria | Nature Communications

Choudhary, E. et al. Gene silencing by CRISPR interference in mycobacteria. Nat. Commun. 6:6267 doi: 10.1038/ncomms7267 (2015).

Hope it helps.

Best,

Johannes Mthembu

Soumajit Mukherjee Thanks so much.