Soumajit Mukherjee

5 Questions 5 Answers 0 Followers

Questions related from Soumajit Mukherjee

Working protocol for Propidium iodide staining for imaging?

Hello researchers, I am trying to do an imaging study with PI for the live/dead cell assay in HeLa cells infected with virus and incubated with nanoparticles as well. Can anyone please share the...

05 November 2020 5,548 1 View

Help in cloning, should I create 5' phosphate group on digested vector?

Hello all, I am trying to introduce an annealed DNA fragment into the double digested vector. The ssDNAs were ordered without a phosphate group modification. The vector is digested with XhoI and...

21 October 2020 8,483 5 View

Fixation method which will not permeabilize the live cells?

Hello, researches, So I am trying to do a cytotoxicity assay with virus-infected and inhibitor incubated cells. I would like to do quantification alongside MTT. I will have to kill the virus with...

21 October 2020 5,264 3 View

Purification of annealed oligonucleotides after annealing procedure?

Hello all, I will do oligonucleotides annealing for the first time which will be later used for cloning without any more modification. Can any of you kindly suggest if I should purify the annealed...

17 September 2020 4,009 3 View

Can anybody give me protocol for mitochondria staining using mitotracker in Huh7 cells?

As the question suggests, I am trying to stain mitochondria in Huh7 cells and the goal will be to visualize only mitochondria using Mitotracker Red CMXRos. So, here I am trying to avoid spending...

02 April 2018 7,461 2 View