Does a parents education level have an impact on their child's education?

Do children follow in the same footsteps as their parents in terms of their education?

12 January 2021 1,375 7 View

Protocol for activating PBMCs using PHA/IL-2?

Hi there, just looking for a simple protocol stimulating 1 x 10^6 PBMCs/ mL in RMPI + 10%FBS using PHA and IL-2? I am looking for CD25, NKp44, c-kit expression and a few other markers for flow...

08 December 2020 2,087 1 View

Any advice on how to get KO cell lines by santa cruz Crispr-cas9 please?

Hello, I tried several times to obtain two KO cell lines for ERN1 (IRE1 alpha) using a santa cruz crispr cas9 "double nickase". (HNSCC cancer cell lines : PECA-PJ41 and PECA-PJ34). I tried...

07 December 2020 8,141 1 View

after dissolving biomass in an ionic liquid and filtering it with denoised water. how may i find out how much of cellulose in my filtrate?

i was wondering if you know of an accurate method for quantifying the amount of cellulose in a filtrate. of biomass that had been dissolved in ionic liquid and then filtered with deionized...

05 November 2020 6,469 2 View

Protocol for fixing mouse livers and fat pads for H&E staining?

Hi there! I need advice on a protocol for mouse tissue staining. I will dissect mouse livers and fat pads and look for liver steatosis and adipocyte perimeter. I was planning on doing this with...

22 November 2019 7,490 6 View

Why do my KGN granulosa cells are shrinking when treated with PMA ?

Hello everyone I need help, I am working with KGN granulosa cell line (DMEM/F12 media, 10% FBS, 1% Pen/Strep) I am treating the cells with PMA at 100nM concentration to activate PKC, but...

29 April 2019 5,057 3 View

Which statistical test(s) should be run with ordinal data?

The research has: 1 IV and 3 DV's. Likert scales used yielding scores of 1-5 on Behavioural Observational Scales (BOS). n=99 with each individual being scored on all 3 DV's. Total BOS=297. 99 BOS...

30 November 2018 8,807 4 View

Which statistical test(s) should be run with what kind of data (nominal, ordinal, etc)?

1 IV and 3 DV's. Likert scales used yielding scores of 1-5 on Behavioural Observational Scales (BOS). n=99 with each individual being scored on all 3 DV's. Total BOS=297. 99 BOS scored for each...

29 November 2018 6,231 4 View

What is the best method of alkaloid extraction from insects?

I'm working on a project for which I need to extract the alkaloids in Lycorma Delicatula (the spotted lanternfly) for HPLC, and I'm having a difficult time finding an adequate protocol for...

14 November 2018 9,004 3 View

Does anyone know any good credible questionnaires measuring social media use?

I am looking for any credible questionnaires that are able to measure a person's social media usage. Thank you.

28 October 2018 2,316 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View