Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

Can you recommend medium recipe for growing aob and nob bacteria in same medium?

hello, I want to grow aob and nob bacteria in the same medium is that possible? if it is can you give me a medium recipe? (what I mean by the same medium is that I want to grow them in the same...

29 December 2020 3,115 1 View

Hi, i'm trying to synthesis Graphene oxide without (NaNO3), how ever at the end of the synthesis it just black dusty solution, any idea?

here is step from journal. 1. add 3g graphite powder in 70ml H2SO4. in ice bath and constant stirring. 2. add 9g KMnO4, after a while it become green 3. heat it to 40celcius for 30minutes, it...

22 November 2020 9,622 3 View

Why my dh5a always flocculated when cultured in M9 medium?

I used the M9 supplemented medium but didn't adjust the pH of 5x M9 salts to 7.2. The final concentration of Ampicillin is 100ng/mL. Here is my recipe: 1X M9 salts 1mM thiamine...

30 October 2020 2,451 1 View

What could be the problem in these pcr results?

All the pictures I have uploaded are gel image of genotyping. What I don't understand is, most of the time, I get very very faint second band, and I assumed it's probably a problem with primer...

28 October 2020 9,410 9 View

Does anyone know a recipe for the PEI (polyethylenimine) buffer?

I am using PEI for my transfection experiments. I used to use the ESCORT V kit buffer, but I ran out of it. Does anyone know the recipe for a buffer that works well with PEI? I really appreciate...

22 October 2020 9,538 3 View

What is the most effective way to transfect HAP1 cells?

I am starting a project with the HAP1 cell line and previously our lab has had difficulty to transfect them. -we have nucleofection equipment - but have only been getting ~10% transfection -i...

19 October 2020 5,340 2 View

Suggestions for transfecting plasmids in ME-1 cells?

Hello everybody, I am currently working with recently purchased ME-1 cells. I was wondering if anyone that has experience transfecting this cell line, or even lines that are similar, would be...

08 October 2020 9,943 2 View