Has anyone used Pro-Touch color bands to mark raptors?

We have been color banding American Kestrels using A-craft alpha-numeric color bands since ~2013 and would like to test other options. I am curious if any other researchers have experience using...

01 February 2021 6,786 3 View

Why have a specified percentage absorbance to be reached for flow test on UV Vis?

When performing a flow test the objective is to find the total amount of blank to be replaced by sample with pump. Why would a specified percentage of 100% i.e. 99.5% of that absorbance be used?

17 December 2020 7,762 3 View

Qiagen Viral RNA extraction kit showed similar results for positive and negative control. How should I proceed?

Our team is looking for viral RNA in the environment. We took swabs off of doorknobs using cotton dipped in water and then submerging that swab in viral transport media. We also wiped 1 surface...

08 June 2020 6,532 3 View

MitoSpy vs. MitoTacker?

I have a very long and rich history using MitoTracker dyes, but I had not, until recently, even heard of MitoSpy. Has anyone used this dye side-by-side with any of the MitoTracker? Is MitoSpy the...

25 November 2019 3,412 2 View

Where can I purchase syndiotactic polypropylene (sPP)?

Hello, I'm looking to purchase syndiotactic polypropylene (sPP). Most articles I find cite purchasing it from Sigma Aldrich, but they sold their last stock in 2013. MilliporeSigma has isotactic...

10 June 2019 6,852 6 View

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08 January 2019 3,512 5 View

How do you construct a crRNA for pCas9 (addgene 42876)?

Hello, I'm designing a CRISPR/Cas9 protocol for school purposes (I attend a extra project next to my study). I have to use the pCas9 plasmid (addgene #42876) for CRISPR eventually. This plasmid...

08 July 2018 5,461 1 View

Is the Pacbio Sequel or the Illumina HiSeq 2500 better for detection of alternative splicing by RNA-seq (next-generation sequencing, NGS)?

We have a biological condition that we believe decreases splicing fidelity in c elegans. In our preliminary RNA seq data using 2x50 bp, 22 million reads per sample and 5 replicates, we found only...

28 March 2018 3,160 3 View

How to reflect human lifespan in rat model?

I am looking to model different ages (in humans) using a rat model. Does anyone have any papers which describe the developmental markers in brains of rats and humans? Example) How many months...

06 March 2018 5,880 4 View

Has anyone compared zero air with nitrogen for nanoflow electrospray of peptides using 5600 with NanoSpray III source?

We are spending more than the cost of the service contract every year on buying 'zero air' cylinders for the electrospray sheath gas. We have the 5600 and 6600 with NanoSpray III sources. We...

31 October 2017 4,279 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View