This cell line is available at DSMZ, Germany, but they do not supply cell lines to India. Can anyone help me to obtain this cell line?
HOW TO WRITE GREEN CONSUMER BEHAVIOUR AS A LITERATURE REVIEW
28 June 2024 2,661 0 View
I needed help to get the published final version of a research article titled: "Alterations in placental long chain polyunsaturated fatty acid metabolism in human intrauterine growth...
11 June 2024 3,120 2 View
need a good reference for designing adsorption column for temperature vacuum swing adsorption. Please suggest
13 February 2024 3,230 0 View
Hi! My lab has recently purchased the immortalised human mast cell line (LUVA). We have reached out to the supplier but have yet to receive a response so I thought I might get some advice on here....
05 October 2023 1,142 0 View
Methanol and ethanol plant extracts of lemongrass, centella asiatica and moringa oleifera not showing activity in the varied concentration of lower to higher concentration 300mg/ml, 500mg/ml and...
15 August 2023 5,375 3 View
HOW to calculate pectinase enzyme activity as, we have values of OD @540 nm obtained from DNS method of different Ph and Temp? please let me know the calculation?
15 August 2023 5,194 0 View
How to calculate pectinase enzyme activity as, we have values of OD @540 nm obtained from DNS method of different Ph and Temp of sub merged fermentation using wheat bran and citrus peel ? please...
15 August 2023 3,422 3 View
Fifty milligram of methanol extract fraction were weighed separately and dissolved in 100 ml of methanol to get 500 μg/ml stock solution. From the stock solution how to prepare different...
06 July 2023 9,223 2 View
Any information regarding the availability of PFGE facility in Tamilnadu?
13 June 2023 7,859 2 View
I have synthesized a Schiff base compound but everytime I do NMR I found an aldehyde peak of intensity 0.20 or 0.25 like that. I have tried column, recrystallization, solvent wash but couldn't get...
29 March 2023 4,641 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View