Ashwini Dhamanage

7 Questions 13 Answers 0 Followers

Questions related from Ashwini Dhamanage

What are the best markers to identify immature and mature dendritic cells from macrophages in mouse BMDC culture?

Hi everyone, I am generating dendritic cells from mouse bone marrow which I am getting >80% CD11c positive cells on day 8. But using GM-CSF alone for DC generation give CD11c positive cells...

06 June 2019 5,074 2 View

What is the appropriate protocol for generation of Dendritic cells from mouse bone marrow..should it be GM-CSF+IL-4 or only GM-CSF?

Hi everyone, I am trying to generate Dendritic cells from mouse bone marrow where I am culturing the bone marrow cells with GM-CSF for 6 days and measuring the percent DCs generated on 6th day by...

08 March 2019 4,662 8 View

Will the standard protein available in ELISA kit be treated as reagent in other assays?

Hello all, I have IFN-alpha kit, which is having IFN-ALPHA protein as its standard. Can this protein be treated as reagent and used as inhibitory or stimulatory agent in other assays like...

19 December 2016 6,562 5 View

What should be time of detection for phosphorylation of IKK-alpha after TLR-7 activation in pDCs?

Hi all, I am working on pDCs in HIV-1 infection. I would like to detect phosphoryaltion of IKK-alpha after TLR-7 activation of pure pDCS by TLR-7 agonist. Afetr how much time should I fix the...

27 September 2016 9,765 2 View

How should I stimulate plasmacytoid dendritic cells to induce phosphorylation of akt?

Hello, I am working on freshly isolated plasmacytoid dendritic cells. I stimulate them with Imiquimod (TLR-7 agonist) to see the phosphorylation of akt (ser473) after 90 min by flow...

20 March 2015 4,805 3 View

What is the best way to attach suspension cells to a glass slide for observation under fluorescence microscope?

I need to observe intracellular protein in my suspension cell line through fluorescence microscopy, but I am not able to get good staining and proper method for attachment of cells to glass...

05 January 2015 8,064 13 View

Can anyone tell me protocol for staining of intracellular IRF-7 for fluorescence microscopy?

I am working on a suspension cell line and I want to observe the intracellular location (cytoplasmic or nuclear) of IRF-7 protein after activation of cells....Please tell me how should I approach...

29 December 2014 4,516 2 View