Ashwini Dhamanage

11 Questions 18 Answers 0 Followers

Questions related from Ashwini Dhamanage

What are the best markers to identify immature and mature dendritic cells from macrophages in mouse BMDC culture?

Hi everyone, I am generating dendritic cells from mouse bone marrow which I am getting >80% CD11c positive cells on day 8. But using GM-CSF alone for DC generation give CD11c positive cells...

06 June 2019 5,123 2 View

How to generate Th1, Th2, Th17 and Treg polarizing /inducing mice Dendritic cells?

Hello everyone, I a trying to generate mice Dendritic cells those will induce T cells into Th1 or Th2 or Th17 or Treg population..what should be the induction cocktail for DCs to get each of...

01 April 2019 2,598 4 View

What is the appropriate protocol for generation of Dendritic cells from mouse bone marrow..should it be GM-CSF+IL-4 or only GM-CSF?

Hi everyone, I am trying to generate Dendritic cells from mouse bone marrow where I am culturing the bone marrow cells with GM-CSF for 6 days and measuring the percent DCs generated on 6th day by...

08 March 2019 4,717 8 View

Will the standard protein available in ELISA kit be treated as reagent in other assays?

Hello all, I have IFN-alpha kit, which is having IFN-ALPHA protein as its standard. Can this protein be treated as reagent and used as inhibitory or stimulatory agent in other assays like...

19 December 2016 6,610 5 View

What should be time of detection for phosphorylation of IKK-alpha after TLR-7 activation in pDCs?

Hi all, I am working on pDCs in HIV-1 infection. I would like to detect phosphoryaltion of IKK-alpha after TLR-7 activation of pure pDCS by TLR-7 agonist. Afetr how much time should I fix the...

27 September 2016 9,828 2 View

Can paraformaldehyde fixed cells be stored for some days to be used for detection of phospho-proteins? and how?

I am working on pure pDC populaion of human origin and I need to detect phosphorylation of AKT after stimulation with Imiquimod. I fix the cells after stimualtion with paraformaldehyde. Can I...

06 April 2016 7,319 6 View

Hello all, Is blocking treatment is necessary during staining procedure of suspension cells to be used for confocal microscopic examination?

I want to stain IRF-7 protein from pure population of plasmacytoid dendritic cells for confocal microscopy...and I am going to use rabbit polyclonal antibody against human IRF-7 as primary...

18 March 2016 2,230 4 View

How should I stimulate plasmacytoid dendritic cells to induce phosphorylation of akt?

Hello, I am working on freshly isolated plasmacytoid dendritic cells. I stimulate them with Imiquimod (TLR-7 agonist) to see the phosphorylation of akt (ser473) after 90 min by flow...

20 March 2015 4,841 3 View

What is the best way to attach suspension cells to a glass slide for observation under fluorescence microscope?

I need to observe intracellular protein in my suspension cell line through fluorescence microscopy, but I am not able to get good staining and proper method for attachment of cells to glass...

05 January 2015 8,115 13 View

Can anyone tell me protocol for staining of intracellular IRF-7 for fluorescence microscopy?

I am working on a suspension cell line and I want to observe the intracellular location (cytoplasmic or nuclear) of IRF-7 protein after activation of cells....Please tell me how should I approach...

29 December 2014 4,558 2 View

Does anyone has MS-5 mouse stromal cell line?

This cell line is available at DSMZ, Germany, but they do not supply cell lines to India. Can anyone help me to obtain this cell line?

05 December 2014 4,956 0 View