Madiha Rasheed I might be able to offer you some help with the vCONTACT2 tool. To begin, it would be great if you could offer additional details about your specific confusion with the input file. However, the vCONTACT2 tool is intended to anticipate and evaluate protein-protein interactions in general.
To utilize the vCONTACT2 program, you will require an input file containing the atomic coordinates of the proteins you want to analyze. This file should be in a certain format, such as a PDB file, which is a macromolecular structure file format. PDB files can be obtained from sources such as the Protein Data Bank.
With your input file in hand, you may use the vCONTACT2 program to anticipate and evaluate protein-protein interactions. The program will provide a number of output files including data on the expected interactions, such as interface residues and interaction energies.
If you have particular questions or concerns about utilizing the vCONTACT2 tool, I recommend checking the program's documentation or contacting the creators.