Does anyone has experience on removel of heavy metals using "Microbial fuel cells (MFCs)" method?

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How long can PBMCs be stored at -70°C with good quality?

Can storing PBMCs at -70°C for a long time affect the population of PBMCs?

15 January 2024 8,010 3 View

Method of measuring sulfur for molybdenum oxide?

What is the method of measuring sulfur for minerals such as metal oxide or molybdenum oxide through Leco furnace (or similar furnaces) at different temperatures, times and weights after leaching...

11 November 2023 3,345 2 View

What is the best cytotoxicity test for car-nk cell evaluation? (with protocol)?

cytotoxicity test

30 July 2023 1,006 1 View

Nursing curriculum and educational system in canada and emarat countries?

i like to know some thing about nursing system in these country something like their salary and other things

06 December 2022 7,604 3 View

How to simulate turbines via EPANET toolkit in Python?

Hi researchers, I utilised epanet toolkit in Jupiter to model a micro pumped storage system. To model the turbine I substitute it with GPV. However, I faced an error when I was about assigning the...

08 November 2022 2,639 3 View

How can i find the rpm of my wind turbine in non rated speed?

my turbine is a variable speed wind turbine.

18 May 2022 7,250 4 View

Hi everyone. Does high concentrations of Tris-HCL buffer ( 2-3 mM Tris in 25 μl real time PCR reaction ) interfere with PCR reaction?

I have dissolved my primers in TE buffer 10X, which in the final PCR reaction will have an EDTA concentration of 0.02 mM and a Tris-HCL concentration of 2-3 mM. Will this interfere with the tag...

14 February 2022 6,667 3 View

How to get consistent result from CD spectroscopy?

I am doing CD studies on amino acids by measuring the CD intensity (quantative) for various concentrations of amino acids both when they are pure and when they have been used as precursors for...

07 December 2021 9,234 7 View

How can I measure electrical conductivity (EC) in mucilage seeds?

I want to measure EC in mucilage seeds. I do not want the seed coat to be damaged. So, how can I measure EC ? Is there another way to replace the electrical conduction method for mucilage seeds?

30 November 2021 6,539 5 View

How to differentiate the emission peak of a compound from the emission peak of the light source(instrument)?

I am having trouble recognizing whether the emission picks that I am seeing are from the light source of the instrument or from my sample?? is there a way to better discriminate them? (I am not...

21 November 2021 9,368 3 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View