Which large sustainable energy demonstration projects are running right now, which have not been reported in a scientific publication yet?

I would like to know which large sustainable energy demonstration projects are running right now, in a country or series of countries, which are too premature to already being subject of a...

03 May 2020 4,174 9 View

R 3D plot with centered axis ?

I want to represent a series of results computed from y = f(x1, x2, x3), with x1, x2 and x3 being in the [-2, +2] range. For now, I use the "scatter3d" package, and an example of a resulting...

21 December 2019 4,986 4 View

How to model proper contact definitions between bodies in ANSYS for rectangular 'pressure vessel'?

Dear all, For a project I am currently working on, the intention is that I calculate the maximum allowable pressure in a rectangular 'pressure vessel'. Of course this shape is not suitable for a...

08 January 2019 7,574 2 View

How do fungicides defeat rust disease?

Also, I would like to ask: 1. How effective are the fungicides? 2. Can rust disease develop resistance? 3. Are there already natural fungicides for rust disease? If you could provide sources to...

16 March 2018 2,135 12 View

What is the effect of dormancy on TTC viability test of embryos?

Will a dormant but living plant embryo always stain red if subjected to TTC? Or can an embryo be so dormant that it will not show dehydrogenese activity?

05 March 2018 7,673 11 View

Does anybody have experience with CATS-small-RNA seq kit from Diagenode?

I am sequencing extracellular vesicle RNA on illumina Nextgen 550, on paper for this kit looks great... but has anybody experience with it?

03 December 2016 2,405 0 View

Does Propidium Iodide have the same fluorescence yield when bound to RNA or DNA?

14 June 2016 8,094 3 View

Troubleshooting Overnight Rest of PBMCs in Serum free Media?

I am working on a protocol to stimulate PBMCs with IFNy to measure transcription activation of genes along the kynurenine pathway using rt-PCR. I want to use serum free media to minimize...

13 June 2016 1,592 3 View

How can I perform mouse cytokine ELISA on high lipid plasma?

Hi All, I'm need to perform an IFNalpha ELISA on mouse plasma samples, however these samples are from mice that received a high cholesterol diet and therefore the plasma is turbid because of the...

11 September 2014 7,970 3 View

Does anyone have experience with global analysis of gene expression, and which method is the best?

I am planning to perform a global analysis of gene expression for the first time. I will be focusing on angiogenic factors. We are using mouse samples. Does anyone have any experience with such...

05 June 2014 5,633 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View