Does anyone has a document about reliable DNA extraction techniques for mycorrhizal colonization?
Methionine, cysteine, homocysteine, and taurine are the 4 common sulfur-containing amino acids, but only the first 2 are incorporated into proteins. Sulfur belongs to the same group in the...
22 July 2024 4,273 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Object-Oriented Programming
29 June 2024 4,877 12 View
Computer Science Department
19 June 2024 8,292 2 View
Various organic feedstocks are used in the thermochemical conversion process to produce biochar. It is essential to analyze the resulting biochar for hazardous compounds to ensure its safe use in...
30 May 2024 6,258 1 View
What is the difference between limitation in recall and recall bias?
19 May 2024 8,812 3 View
One of the promises of the Holy Qur'an is the inheritance of the earth to the oppressed, according to the verse, " And We desired to show favour unto those who were oppressed in the earth, and to...
10 April 2024 3,604 0 View
I am trying to isolate exosomes from the cell culture supernatant of Adipose Mesenchymal Stem Cells (20-30 ml) by the PEG isolation method. But exosome pellet is not visible after isolation. DLS...
08 April 2024 3,879 1 View
No informations furnished from the supplier. I am working with this type of resin for the first time! In litterature, I found these steps: - Use deionised water to remove salts until neutral pH...
12 March 2024 4,612 0 View
Hello. I am making hollow nanofibers using electrospinning technique and coaxial needle. My efforts have not been successful so far. I use PVA polymer for the shell and mineral oil for the...
22 February 2024 3,064 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...
05 August 2024 8,025 7 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View