How many mice are needed for long term potentiation?

As above, we are trying to do the Long-term potentiation test using mice hippocampal slices. How many mice are needed or how many slices are needed for LTP? Can anyone help me with that?

15 February 2020 7,073 3 View

ARIMA, R square and stationary R square?

Dear all When ARIMA model was used to make the unstable time series data stable. There are many model to choose. which is the best one? Not only BIC value was shown, but also R square,...

24 December 2018 7,782 3 View

Aberrant protein after crispr/cas9?

Hi All, I used Crispr/cas9 to knockout CISH in T cells. The T7EI assay showed the gene editing. However, when I used the Western blot to check the protein expression, I found the the bands with...

12 November 2018 3,755 4 View

Is there any textbook or technical instructions on the preparation of ceramic powder and sample?

Dear all, I am to do some experiment on ceramic powders, alumina for example and wondering how to prepare the powder and sample. I wish to have some recommendations on textbooks and technical...

04 March 2018 4,077 4 View

How to stain RNA for EM?

Does any one know a good reagent (or protocol) to stain nucleic acids (RNA) for electron microscopy (either negative stain or positive stain)? p.s. This is for single particle analysis. Thanks!

31 October 2017 3,663 3 View

HOW TO DO ATRP REACTIONS ON THE SURFACE OF SiO2 NANOPARTICLES?

I encounter two questions during SI-ATRP. My experiment route is shown as follows. First ATRP reaction, MSN-Br(100mg), GMA( Glycidyl methacrylate, 1.5mmol),...

23 December 2016 3,066 3 View

Are deep neural networks practical to problems with low-dimensional input data(e.g. under 10 dimensions) ?

Deep neural network has been proved that efficient in imaging, text and voice data. Are they work good in processing low dimensional data ? (One example, using the DNN to analysis mechanical...

19 May 2016 3,367 3 View

How to do protein purification by intein tag/chitin beads in mammalian cells?

17 April 2016 1,304 2 View

What's the difference between the polarization direction, maximum radiation direction and direction of the electric field distribution?

10 February 2016 7,955 2 View

Is measuring Bradykinin levels in plasma possible?

Hi, does anyone have the experience in measuring bradykinin (BK) level in samples (plasma, medium etc). Since BK is easily degraded to its metabolites in vivo, I think elisa kits that only react...

29 December 2015 6,350 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View