we are performing intracraneal injection of in NOD SCID Gamma mice, and we are finding unusual problems with anesthesia, loosing 75% of mice during surgery or next following days. From last 8 mice, only 1 survived. We can not imagine why, so any advise will be wellcome!!!
Thanks a lot.
I have not used ketamine for anesthesia primarily due to its toxicity. I usually prefer using 3% avertin solution. If you standardize its use, the recovery of the animals are really good with this. I have kept them for atleast a week without any complications following the anesthesia.
Hope it helps.
http://web.jhu.edu/animalcare/rdf/avertin.html
http://cshprotocols.cshlp.org/content/2006/1/pdb.rec701.full?text_only=true
Yes! NSG mice are very susceptible to ket/xyl anaesthesia. The best is to try reduce your dose to 80% what you usually use per body weight and then bring mice back immediately after procedure with atipamezole (Antsedan) at 1mg for every 10mg Xyl administered.