Can anybody know extraction method for total cardiac glycoside estimation. But it should be crude method. or else spectrometer method but as per i know standard for spectrometer method is too costly. please suggest me proper solution.
Please see following papers:
https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwjF06vz68_QAhWGpo8KHdbADJcQFggiMAA&url=http%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2F10.1111%2Fj.2042-7158.1953.tb14006.x%2Fpdf&usg=AFQjCNFNW69JZevu1Wiwbf8I_STzqMtmUQ&sig2=PEfrlcCVl6o4L2qRSHJQng
http://www.rjpharmacognosy.ir/article_13771_2f6033a40df8b79102a55d6aac176b52.pdf
https://www.ncbi.nlm.nih.gov/pubmed/13184433
Thanks sir for ur reply,
I already refer this paper but in that i found preliminary estimation. I want to know total amount in my plant. let me know if you have any reference for quantitative estimation.
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