Does anyone could suggest a reliable BMDM immortalization protocol?
I use SV40 transfection to immortalize MEF cell and brown-fat drived SV cell. In theory, this can also be used for BMDM immortalization.
I looking for topic related information.
22 January 2022 8,012 4 View
I'm looking for any smart villages aplications in your country. I'm interested in examples that involve the enviroment, agricultural, society and technology. I would be grateful for any input.
03 August 2021 8,385 10 View
I am looking for research on the topic indicated. I would be grateful for your help. I am mainly interested in the dichotomy of feelings, subjective poverty and deprivation of needs.
12 April 2021 772 12 View
Have any of you tried creating an index of subjective poverty, but other than LPL or SPL?
19 January 2021 7,577 4 View
I have recently carried out research on how academic teachers from Central and Eastern European countries universities (from Poland, Slovakia and Hungary) now understand "making a scientific...
17 May 2019 8,781 3 View
Dear All, I'm looking for the picture of the template structure (example of oligonucleotides) for crRNA synthesis by transcription in vitro by use of HiScribe T7 Quick High Yield RNA Synthesis...
25 April 2019 1,313 0 View
I am looking for a free software to support calculating the added masses and damping matrices of the ship.
10 December 2018 3,369 1 View
Hi! I would like to discriminate dead cells from my population using LIVEDEAD fixable dye. In principle it stains amine residues in both live and dead cells, but because of cell membrane...
28 October 2017 5,374 3 View
I read few books, including Beverly J. Silver on global proletariat (and automotive industries), but while writing about private automobilism from Marxist perspective and still feel, that...
28 June 2017 1,479 6 View
Unfortunately, I am well aware only prof. Bauman's text in Polish...
18 June 2017 235 3 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View