Anyuan He

2 Questions 3 Answers 0 Followers

Questions related from Anyuan He

Inducible knockdown two genes in MEF cell?

Hi guys, Recently, I try to knockdown two genes in MEF cell by using tet-induced knockdown lentivirus provided by this paper (attached). several steps are involved. 1. Cut the...

26 November 2016 8,993 0 View

The deletion of my studied protein increase the total DNA, RNA and protein level in MEF cell, some one can give me some suggestions?

Hi all, as described in the title. I isolate genomic DNA, total RNA and total protein from WT and X1 gene KO MEF cell. I am surprised when I know the concentration of the sample. The initial cell...

14 July 2016 2,643 4 View