I have my blood in Paxgene RNA tubes. I'm quantifying with nanodrop and the kit handbook is not clear about the blank to used for nanodrop (to do a dilution in tris-hcl 10mM is what they recommend in the some proportion for the sample and the elution buffer (blank); but the results are similar and I think more correct if I just used the elution buffer to do the blank.)
My ratio 260/230 are about 0,33-0,68 , really low. I want to use it for RNAseq
Best regards,
Thanks in advance,
Mariana
For RNAseq do not quantitate by nano drop, go straight to a Bioanalyzer. We also had low 260/230 of about 0.5 for microRNA isolated by Qiagens microRNA kit.
Yes. I increased the centrifugation of the colun before elution and it seems that is getting better.. Yes i will try. We don't have available in our center. When you used it, in the step that you add isopropanol, I see fibers. Should I pass them to the next column or not?
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