Does anybody know, what is the way to find promoter region of the non-coding microRNA gene?

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Have anyone any idea how mirStart (online microRNA promoter region prediction tool) scored intergenic microRNA promoter region ?

http://mirstart.mbc.nctu.edu.tw/browse.php or http://mirstart.mbc.nctu.edu.tw/mirna.php?no=55892 Actually, miRstart online tool predicted promoter region characterized by CAGE tag, TSS tag, and...

11 December 2017 7,101 0 View

Please can anyone suggest me, how can I knockdown my target microRNA, which is expressed in the basal level into my cultured cell line?

I am trying to knockdown basal level expression of my target microRNA by transfected Anti-Mir,miRNA Inhibitor, but I didn’t get significant level of knockdown result by real time PCR. Have any...

08 September 2016 2,147 1 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

Is there any way to calculate whole genome similarity of organism without the whole genome sequencing ?

is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?

05 July 2024 4,070 3 View

CCN concentration over Arctic region?

I need CCN concentration over Arctic region, any observational dataset is welcomed. Thank you.

01 July 2024 6,082 0 View

Is it okay to grow bacteria in 30 for cloning? What is the lowest mass of Vecor I can take for cloning? what should be the optimum insert ratio?

I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...

26 June 2024 3,735 3 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Why is the blue and white spot screen negative for all white spots?

The protein was expressed using the insect cell system, and the recombinant plasmid was successfully constructed as verified by sequencing, and the recombinant plasmid was transformed into DH10...

13 June 2024 2,590 4 View

I want to design a study for ex situ conservation of rare ferns based on ecological and genetic diversity. what could be possible method?

I want to design a study for ex situ conservation of rare ferns based on ecological and genetic diversity. what could be possible method other than cryopreservation of spore for a short term...

10 June 2024 4,992 1 View