Trying to transfect OVCAR3 cells with a RFP reporter+puromycin resistance marker. Ideally, I would run a kill-curve experiment, but a deadline is quiclky approaching. Literature search has yielded only one paper, according to which the dose is 5 ug/mL (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065197/). Have anybody else treated OVCAR3's with Puro? Thank you.
Rashid Gabbasov
Missed the answer. Thank you, Claudia. I had to run the kill curve btw, and the mld turned our to be 0.5 ug/ml
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