I am interested from a behavioural perspective as owners commonly report that dogs do no seem to like people wear high visibility clothing.
I did an SDS-Page and afterwards a Western Blotting to detect IgY form egg yolk of chicken eggs. One sample was boiled and the other was not. In my SDS-Page, there were for the boiled sample bands...
13 May 2024 5,145 2 View
I am planning an immunoprecipitation experiment using Mouse monoclonal [11C9] to Mannan Binding Lectin/MBL (ab26277) antibody to immunoprecipitate for MBL2 in human serum. There are many protocols...
26 March 2024 3,510 4 View
Working with materials and materiality in design processes my reserach has moved from sustainable and circular design towards regenerative design.
22 February 2024 9,695 0 View
I have the data of every avian species I saw at 38 study sites within one month, to calculate the biodiversity of each study site should I use Simpsons index or Shannons Index? or another one?
20 February 2024 6,782 7 View
Hi All, I have recently acquired a ThermoFisher Automated Cell Counter (Countess 3). I am trying to input the parameters/gating for the cell line BHK-21 for the machine to identify live and dead...
13 February 2024 5,213 0 View
I am facing a weird reversal of two positively related constructs (positively correlated, path positive when no other variables are present in the model) within the full structural model...
20 November 2023 1,590 5 View
I aim to validate a formative construct (GPers) and I put it in a model with two reflective constructs (CI and Willing) to do so. AMOS gives me estimates in the output, but they don't appear in...
16 October 2023 7,419 4 View
I have carried out a PCR reaction, resulting in a product of ~227bp. I quantified the raw product using PicoGreen, while this isn't entirely accurate with other components potentially interfering,...
04 September 2023 8,691 5 View
We wish to invite members of the public to submit certain types of videos for a research project. The videos are likely to be too large for people to be able to email to us. Has anyone found a...
03 July 2023 1,610 2 View
Which buffer is best for Benzonase treatment of thawed PBMC? I want to prevent cell clumping, not cause cell lysis. I can find lots of details of how much to dilute benzonase ol for this purpose,...
02 July 2023 8,112 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
What is the method for analytically calculating the wear and service life of polymer-polymer sliding pairs?
04 August 2024 1,078 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....
31 July 2024 3,843 7 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.
29 July 2024 6,115 0 View