I am interested from a behavioural perspective as owners commonly report that dogs do no seem to like people wear high visibility clothing.
Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...
25 February 2021 9,659 2 View
We recently have been moving away from using agarose gels and onto using the Tapestation (D1000 for PCR products). However, we have noticed that there are a lot of extra bands that show up on the...
15 February 2021 791 3 View
I'm looking to compare a few fluorescent dyes for cell permeability. I'm interested to see how much of the dye actually enters the cell, but would specifically like to quantify this data. Any...
21 January 2021 6,089 3 View
I would like to pharmacologically increase presynaptic neurotransmitter release in mice CNS in vivo (through the extracellular micro-infusion of a compound). Which strategy would you recommand?...
02 November 2020 5,645 3 View
I have a custom plasmid which expresses mCherry and will act as a reporter. I can transform this plasmid into DH5a and BL21. With DH5a I get a good number of singular colonies, but with BL21 I get...
02 September 2020 9,063 3 View
Hi there, we try to establish a multiplex western blotting method using fluorescence-conjugated sec. antibodies (anti-rabbit IgG StarBright Blue 520 and anti-mouse IgG StarBright Blue 700 from...
27 August 2020 7,787 5 View
Hi All, I am looking for recommended valid measures/questionnaires to measure a change in psychological flexibility. To be delivered pre and post intervention. Can either measure psychological...
18 August 2020 8,187 3 View
Hi there! I am currently writing up my MSc oncology dissertation; I have 5 cancer cell lines, 1 responds to a certain treatment, the other 4 do not. I am trying to find a statistical test which...
09 July 2020 3,720 4 View
Good morning all, I am having some trouble interpreting my regression output. My model is LN of transaction volume = alpha + beta1_RER + control variables RER is defined as the change in the...
28 April 2019 516 3 View
Part of my dissertation looks at a replication of the NEP study conducted in 2000. I'm attempting to compare the two (my repsonses and the responses received in 2000) and see whether my survey...
06 March 2019 4,050 6 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...
01 March 2021 1,960 4 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...
28 February 2021 2,393 8 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Hello, I created a profile for my friend on google scholar. I have added a verified email address. I marked the profile as public. Formerly his profile was visible. However, now, When I type his...
24 February 2021 8,680 3 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...
24 February 2021 2,006 2 View
I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....
21 February 2021 8,609 1 View