I'm trying to deplete human sera of visfatin. I have had success with neutralizing antibodies for other cytokines in the past such as IL-6.
The study involves age-specific range (15-16).
06 May 2024 2,756 2 View
i am doing my masters in criminology and currently doing an assignment on the effectiveness of the tcs and was querying the difference if any on the private sector run dovegate or the state one...
27 March 2024 3,380 0 View
How can I simulate a UASB reactor UASB in ASPEN PLUS to remove COD and BOD?
10 October 2023 9,534 0 View
I am looking at tidally influenced groundwater at Longview, WA. This is 67 miles upriver from the mouth of the Columbia River but there is tidal influence.
01 October 2023 9,308 13 View
Good morning, I would like to know a protocol for in vitro cultivation of avocado, I have no experience in that area, but I must obtain in vitro plants free of fungi, that is the idea. if anyone...
14 November 2022 4,520 0 View
I have been working on acidified silicas and found that bare mesoporous SiO2, when refluxed in 12M HCl, is apparently very active in the reaction I'm looking at. With further testing on the...
13 April 2022 7,135 3 View
Hello, I`m working with an ATR-FTIR probe for continous concentration measurement during crystallization. Fouling of the probe tip is a problem that I observe in most of the experiments. Does...
06 January 2022 3,809 3 View
my group has a small vacuum chamber built for thin film co sputtering, the two guns were initially coupling but we solved that issue by spacing the guns farther apart. However, the issue with the...
22 October 2021 5,692 4 View
I'm trying to find a good internal standard for aqueous Fructose dehydration to 5-hydromethylfurfural, for use in an HPLC (due to the water). It doesnt appear any studies actually use an internal...
20 August 2021 9,623 1 View
I'm new to enzymes and I would like to receive all possible help for this. I have a product that initially contains 57.9g of starch, which I would like to hydrolyze with alpha amylase, that has an...
25 March 2021 2,772 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....
31 July 2024 3,665 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View