How to prevent probe fouling of ATR-FTIR in a crystallization process?

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Why do I get no result from Mauchly test in SPSS?

Hello, I have a statistical problem I need help with. But I can't find it anywhere I did a test with 31 subjects and 3 treatments. Each subject underwent each treatment in a randomized manner. I...

23 July 2018 8,979 10 View

Is the E-test on SAB agar for the Scedosporium apiospermum reliable?

I had to perform a MIC test on Scedosporium apiospermum last week (isolated from a BAL). Unfortunatly I couldn't use the specific and required media I was suppose to (potato's agar to select the...

17 March 2015 2,326 5 View

I have been trying to develop a HPLC method for Meropenem trihydrate but failed in many attempts. Are there any suggestions?

I have tried the following mobile phase solvents and ratios of them. I am using 1ml/min FR and 298nm WL. Either I do not get the peak, it is too near the solvent peak, peaks not reproducible or...

04 October 2012 5,973 35 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

How to build fouling and erosion simulation model for Aero Engine compressor and turbine rotor in Simulink / Matlab?

22 February 2021 3,697 1 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

How can I label an antibody to determine how far it is travelling from the microdialysis probe?

I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...

09 February 2021 3,981 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

How can I get access of UNAfold tool?

I am searching for tools to construct molecular beacons which shall be complementary to a given sequence. I don't want to design any primers. I found that UNAfold is one such tool which I could...

02 February 2021 9,065 5 View

Is there a comprehensive database for Virus Real-Time Primers and Probes ?

Dear All I am looking for a database for tested Real Time PCR primers and probe sequences for the following viruses for diagnostic purposes. Does the USAID PREDICT project or the Global Virome...

01 February 2021 8,920 1 View

How to find primers for HLA DQB105031, DRB11405 and DQA1*0104? for PCR-SSO technique?

I am currently doing research about association of HLA DQB1*05031, DRB1*1405 and DQA1*0104 with rheumatic heart disease population in Indonesia as pilot study (as Koyanagi did in 1996 for...

16 January 2021 7,052 0 View