How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

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26 December 2020 1,951 3 View

How to determine the total and specific IgY?

Comparing the results of two ELISAs We are working with a solution of purified egg antibodies (IgY), both specific (polyclonal) for certain microorganism and non-specific. The problem is, we want...

12 August 2020 3,327 2 View

Why is my bacteria not growing after a transformation?

Hello, I did a transformation a few weeks ago and while only a few bacteria grew, I was able to pick single colonies for a liquid culture. However, I had to repeat this experiment because...

28 July 2020 278 6 View

Any suggestion of bibliography in a wide sense (papers, guidelines, systematized experiences) about "male" parenting (fathering)?

Any suggestion of bibliography in a wide sense (papers, guidelines, systematized experiences) about "male" parenting (fathering) and in special in the context of children and adolescents with...

23 February 2020 8,306 3 View

How to derive reflectances from a Calibrate Panel?

I am working with a hyperspectral camera (Headwell) and I get the DNs from it. I don't have any calibration files of the camera. Do you consider that is possible to estimate reflectances using a...

18 November 2019 2,915 1 View

Why is my tissue detaching after H&E staining?

Hello! I've been having trouble with staining lung and pancreatic tissue. While doing H&E, tissue partially (or completely) detaches. Sometimes, the tissue folds on itself as well. Not sure why...

31 October 2019 8,432 4 View

How can I claim authorship of publications wrongly assigned to another person?

A person with my same surname appears as the author of several publications authored by me.

05 June 2019 7,712 3 View

From whole mitochondrial DNA sequencing data of paired peripheral blood /tumor samples, which is the best algorithm to identify somatic mutations?

We are using SRELKA to identify somatic mutations from whole mitochondrial DNA sequencing data of paired peripheral blood /tumor samples. However, we have noticed that the somatic mutation list...

12 August 2018 1,406 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View