Does anybody have the sequence of plasmid pLEX MCS?

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Is there a lot of difference between standard LFP batteries and the LFP used in ev cars?

Apart from the size I can´t find any information about the LFP batteries used in BYD ev, for example. Can the size affect the discharge of tha battery or other characteristics?

19 March 2024 2,229 1 View

¿Cómo se aborda metodológicamente desde el trabajo social el estigma y la discriminación asociados a las drogodependencias?

Con esta pregunta se pretende identificar los métodos y técnicas más adecuadas para abordar las razones por las que las personas drogopendientes totalmente rehabilitadas, se encuentran en...

10 March 2024 6,561 2 View

Bad quality results in Sanger sequencing?

Hello everyone. I am struggling to get good quality results on my Sanger sequencing reactions. I don't know the specific problem that I am facing. In a single order, most of the chromatograms...

03 November 2023 4,035 0 View

Hello community! For those doing experiments with retinal in plants, Do you grow your plants in darkness during the retinal treatment?

Since retinal is a photosensitive compound, I wonder whether you perform your experiments in darkness or do you allow certain amount of light. I've read some experiments for root development and...

15 October 2023 4,863 0 View

Currently, easily extractable glomalin is still a relevant factor in studies on the formation of stable aggregates?

The relevance of easily extractable glomalin in studies on the formation of stable aggregates is still a subject of scientific debate. While easily extractable glomalin has been used as an...

11 July 2023 6,963 2 View

Biodegradable packaging for liquid food products?

Hi, I am looking research institutes or companies specialized in plastics because we would like to explore collaboration opportunities and learn more about the projects of biodegradable packaging...

22 June 2023 6,619 0 View

Pipelines inspection in subfluvial crossings?

Hello, someone can help me with information about technology, device or methodologies for inspection, monitoring and repair of damage to pipelines located in subfluvial crossings, pipe installed...

06 March 2023 2,340 4 View

Could two different compounds has the same retention time in HPLC-RP ?

I'm trying to separete by HPLC-RP veratryl alcohol from its oxidation products, however, as the oxidation occurs the chromatographic peak for veratryl alcohol increases with the time contrary to...

28 November 2022 8,900 0 View

How to compress a simulation box in LAMMPS??

I'm trying to compress the size of a simulation box in lammps, I'm using Reaxff as the force field because I have a large system of clusters of aromatic layers (C,H,O,N) To compress the box, I'm...

15 November 2022 1,848 1 View

Can I add something to the deionized water during washing chitin to obtain faster a neutralized chitin?

I am trying to obtain chitin by a chemical method. After deproteinization and demineralization processes, the powder obtained after each step is too alkaline or acidic respectively. Then, I spend...

10 July 2022 1,686 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View