Why are mouse Renal Proximal Tubule Epithelial Cells (RPTEC) turning to fibroblasts?

I am cultivating RPTEC isolated from mouse renal cortex and immortalized with temperature sensitive SV40 (tsA58) in DMEM:F12 with 2% certified FBS (Gibco 16000-044), 2 mM L-Gln, 1x final insulin...

08 August 2019 3,424 3 View