Difference in endogenous control expression and no sample left. Can the data be salvaged?

16 April 2024 0 6K Report

I have two groups that I collected microglia from and did RTqPCR. However, the CT values come up as extremely different (p

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More Yonaida Valentine's questions See All

What are the necessary step and topics on how to break down your full report into a paper for publishing?

Hello, I wish to know how to break down a full report into a lesser number of pages that will fit publishing rather than publishing the full report of about 200 pages. So, what topics or main...

19 November 2020 5,990 7 View

Is there a need for the reliability of intsurments to be presented when submitting a grant proposal?

Our research project has been selected for funding and we are expected to provide a detailed proposal of the work. I am particuklarly confused if we should state how the reliability would be...

18 October 2020 4,702 4 View

Is it a good practice to publish in a journal, an article that appears in a conference proceeding?

Which of these two statements represents an acceptable practice? A. Publishing a paper that appears in conference proceedings in a journal is okay B. It is okay to publish a paper presented at a...

31 July 2020 3,633 5 View

Possible ways to separate two bioactive compounds obtained from a silica gel column that two dimentional tlc plate chromatogram has shown as isomers?

Compounds were obtained from silica gel column in very small quantity which could not be separated by prep tlc or on another column because one is not sure of getting anything at the end

11 November 2019 6,396 4 View

In CFA, Can I correlate error terms of different latent factors as suggested by modification index in AMOS?

I am using AMOS and have performed a CFA but my model has not achieved fitness. The modification index suggest correlation of several error terms. However, one of the pair of error terms is from 2...

09 September 2019 7,179 13 View

What are the problems in the sustainability of graduate programmes in Universities these days?

I am trying to present a problem of graduate programmes sustainability. However, i am a bit confused on what constitute poor sustainability of graduate programmes such as Masters and Ph.D. programmes

04 June 2019 8,727 7 View

Should single author papers be scored the same as papers with multiple authors?

During assessment, authors are usually scored based on their position in the list of authors for papers published. This is used for decision making and performance appraisal in Nigeria. Given the...

02 April 2019 7,523 16 View

Is it possible to run a moderation analysis using PROCESS by A Hayes in SPSS with a multidimensional moderator?

I would like to know if it is possible to run a moderation analysis with a multidimensional moderator in SPSS using the PROCESS macro

26 November 2017 7,189 1 View

Does anybody have a good antibody to detect human cells in mouse xenograph model?

my cells are human breast cell line GFP/luc but my GFP signal is very low. I cannot find a good anti-GFP to detect it (I also tried TSA amplification, it is not working). Anti-Luciferase antibody...

09 December 2014 2,156 19 View

Is there any bromelain from certain types of pineapple which is unstable (labile) at low temperatures but more stable at high temperatures?

I'm doing characterization of optimum temperature for bromelain enzyme from Bogor pineapple by measure the activity of the enzyme. I used casein 1% as substrate dissolved in universal buffer pH 8....

03 July 2012 3,380 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View