Difference in endogenous control expression and no sample left. Can the data be salvaged?

16 April 2024 0 6K Report

I have two groups that I collected microglia from and did RTqPCR. However, the CT values come up as extremely different (p

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What will be the objectives of this topic; the impact of relationship marketing on student satisfaction in teesside university?

help me the avail research objectives

27 March 2023 5,431 1 View

Can anyone help to convert from molecules/cm2 to micrograms/m3?

I am working on TROPOMI/ NO2 data which is in the molecules/cm2 unit, I need to convert the molecules/cm2 into microgram/m3.

11 October 2021 4,797 4 View

Can a study have more than one population unit? Put differently, can a study have multiple population?

A friend of mine is conducting a study on "Principals' managerial skills and School effectiveness". The dependent variable of the study is school effectiveness. The researcher intends to find out...

27 September 2021 3,851 5 View

What are the necessary step and topics on how to break down your full report into a paper for publishing?

Hello, I wish to know how to break down a full report into a lesser number of pages that will fit publishing rather than publishing the full report of about 200 pages. So, what topics or main...

19 November 2020 6,244 7 View

Is there a need for the reliability of intsurments to be presented when submitting a grant proposal?

Our research project has been selected for funding and we are expected to provide a detailed proposal of the work. I am particuklarly confused if we should state how the reliability would be...

18 October 2020 4,869 4 View

Is it a good practice to publish in a journal, an article that appears in a conference proceeding?

Which of these two statements represents an acceptable practice? A. Publishing a paper that appears in conference proceedings in a journal is okay B. It is okay to publish a paper presented at a...

31 July 2020 3,791 5 View

How can I model individual parameters of the Hershel-Bulkley model with the Arrhenius equation?

I am studying the rheological behaviour of some pastes at different temperatures. A common approach to modelling the influence of temperature is to consider its effect on the apparent viscosity of...

16 January 2020 8,808 3 View

Possible ways to separate two bioactive compounds obtained from a silica gel column that two dimentional tlc plate chromatogram has shown as isomers?

Compounds were obtained from silica gel column in very small quantity which could not be separated by prep tlc or on another column because one is not sure of getting anything at the end

11 November 2019 6,566 4 View

In CFA, Can I correlate error terms of different latent factors as suggested by modification index in AMOS?

I am using AMOS and have performed a CFA but my model has not achieved fitness. The modification index suggest correlation of several error terms. However, one of the pair of error terms is from 2...

09 September 2019 7,409 13 View

What are the problems in the sustainability of graduate programmes in Universities these days?

I am trying to present a problem of graduate programmes sustainability. However, i am a bit confused on what constitute poor sustainability of graduate programmes such as Masters and Ph.D. programmes

04 June 2019 8,892 7 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Do you think can be any diamond in A type eclogites?

I want to know more about diamond ore deposits in world.

08 August 2024 1,514 0 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,706 0 View

Enhancing Critical Thinking Skills for Slow Learners: A Review of Empirical Studies?

to identify themes in question with APA style references

07 August 2024 2,239 5 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Do you think can be any gas and oil bearing rocks in Eastern part of Iran?

I want to know more about petroleum deposits in Iran.

02 August 2024 8,725 3 View