Does anybody have experience with the use of gemcitabine (Gemzar or a generic product) in non human primates? What would be the correct dose?

More Geert C. Mudde's questions See All

What reference wavelength should be used when calculating the spectral slope from CDOM absorption data?

I'm trying to understand the best way to calculate the spectral slope from absorbance data on dissolved organic matter (DOM). The following formula can be seen in a lot of articles (e.g. Helms...

06 December 2022 279 0 View

Can you help me with a protocol for in vitro/ex vivo stimulation of CD8 T cells using peptides?

Can I use short peptides (8-12) amino acids which will bind to MHC Class I directly? and/or do I need large peptides which are internalized and presented in the conventional way? Which is the more...

10 July 2022 1,254 0 View

Does anyone have more information on the geomagnetic storm of march 13th, 1989 and it's impact on the electric grid?

Hello, Does anyone have information on that geomagnetic storm and it's impact/influence on MV, HV and/or EHV electricity networks? Input will go to a group of Belgian Experts on HV/EHV networks...

27 October 2020 7,104 3 View

If you had an article that assesses the validity and reliability of a certain test with only 2 assessors, would you think that is enough?

If you had an article that assesses the validity and reliability of a certain test with only 2 assessors, would you think that is enough to make a statement concerning the intertesterreliability...

29 December 2017 1,170 14 View

Im studying the procedé of pressing woodfibre pallets. What is the amount off pressure (BAR,KG/CM2,PSI) and temperature and duration for good results?

I want to test al possiblitys of pressing cellulose containing materials (wood, fibre, weed, paper and so on) with a heeted hydraulic pressing machine. I would like to translate this possibiltys...

09 October 2017 6,299 0 View

TBA contamination, anyone has a cure to get it out of your UPLC?

Hi, I have been doing some ion pairing chromatography, with TBA (tributylamine). Seems now that when i measure in ESI+ there is a huge background of TBA (m/z 186.2) and i can't get it out of the...

05 September 2017 3,874 6 View

Algorhythm that predicts risk for stroke and heart diseases

Hello, I am looking for an algorhythm that predicts risk for stroke and heart diseases while it compares it to the risk of taking the usual medication. Say I have one riskfactor in six and want to...

23 May 2017 9,531 6 View

Can anyone recommend a practical guide to co-word analysis?

Co-word analysis is a method for tracing developments in a scientific discipline on the basis of texts. Often, keywords are used but other source materials, such as title words, are also used. I...

20 February 2015 5,958 3 View

Does anyone have experience with the synthesis of meso-mandelide?

I'm using the protocol of Tianqi et al. (dimerisation of mandelic acid by 3 days of reaction in mixed xylenes while removing water byproduct by means of a Dean-Stark trap), but for some reason, on...

16 March 2014 9,515 5 View

Does anyone have experience with the synthesis of meso-mandelide?

I'm using the protocol of Tianqi et al. (dimerisation of mandelic acid by 3 days of reaction in mixed xylenes while removing water byproduct by means of a Dean-Stark trap), but for some reason, on...

02 March 2014 8,003 5 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View