Can I use short peptides (8-12) amino acids which will bind to MHC Class I directly? and/or do I need large peptides which are internalized and presented in the conventional way? Which is the more efficient method?
I'm trying to understand the best way to calculate the spectral slope from absorbance data on dissolved organic matter (DOM). The following formula can be seen in a lot of articles (e.g. Helms...
06 December 2022 279 0 View
Hello, Does anyone have information on that geomagnetic storm and it's impact/influence on MV, HV and/or EHV electricity networks? Input will go to a group of Belgian Experts on HV/EHV networks...
27 October 2020 7,104 3 View
If you had an article that assesses the validity and reliability of a certain test with only 2 assessors, would you think that is enough to make a statement concerning the intertesterreliability...
29 December 2017 1,170 14 View
I want to test al possiblitys of pressing cellulose containing materials (wood, fibre, weed, paper and so on) with a heeted hydraulic pressing machine. I would like to translate this possibiltys...
09 October 2017 6,299 0 View
Hi, I have been doing some ion pairing chromatography, with TBA (tributylamine). Seems now that when i measure in ESI+ there is a huge background of TBA (m/z 186.2) and i can't get it out of the...
05 September 2017 3,874 6 View
Hello, I am looking for an algorhythm that predicts risk for stroke and heart diseases while it compares it to the risk of taking the usual medication. Say I have one riskfactor in six and want to...
23 May 2017 9,531 6 View
We are planning a chemo+vaccination combination study in NHP to mimic potential use in pancreatic cancer patients. The human clinical dose of gemcitabin is 1000mg/m2.
26 March 2015 8,450 2 View
Co-word analysis is a method for tracing developments in a scientific discipline on the basis of texts. Often, keywords are used but other source materials, such as title words, are also used. I...
20 February 2015 5,958 3 View
I'm using the protocol of Tianqi et al. (dimerisation of mandelic acid by 3 days of reaction in mixed xylenes while removing water byproduct by means of a Dean-Stark trap), but for some reason, on...
16 March 2014 9,515 5 View
02 March 2014 8,003 5 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,543 11 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View