Paul Rios

3 Questions 5 Answers 0 Followers

Questions related from Paul Rios

Calculate a colony count?

For example, if I put 30uL of a bacteria suspension(dil 10-3) and I count 25 colonies at a agar plate. The CFU/mL will be (25x1000/30)x103? Is that ok?

11 November 2016 9,631 11 View

Which is the best way to store Pseudomonas aeruginosa isolates?

Hi, In our lab we store P. aeruginosa isolates in Tripticase soy broth with 15% glycerol at -80oC, but almost when we culture those stored isolates they do not growth or only are a few colonies....

05 May 2016 623 13 View

Does anybody have experience with Mzia 1 and Mzia 2 primers to identify G23 capsid of Myoviridae family?

Hi! I'm starting to standardize the PCR, so I'm not really sure wich is the best DNA concentration or primers concentration that I need to use Mzia 1(5' GATATTTGIGGIGTTCAGCCIATGA 3') Mzia 2(5'...

07 July 2015 9,905 7 View