Another solvent different to DMSO and Ethanol for picrotoxin?

Im working with drosophila, and i want to avoid those solvent because the influence the may have in memory.

11 December 2019 3,638 2 View

Appetitive or aversive training for drosophila learning?

I want to know if there are any differences between the 2 types of training. I aprecciate any suggestion or literature. aditionally how can i asses appetitive learning in a t maze elevator

01 February 2019 7,712 2 View

Electric shock or mechanical stimuli for conditioning in drosophila?

I want to know if the mechanical stimuli could cause any mechanical lesion or damage in wings or others structures that later mmay affect the perfonmance in the t maze

01 February 2019 9,262 2 View

How to prepare L15 medium for insect culture?

I want to culture honey bee neurons, the methods include to supplemented it with sugars, I want to know if after added sugars to the medium is recommend to filtered o sterilized again. orhow its...

10 November 2018 9,672 1 View

How to evaluate statistical diferences when theres no control data?

Im evaluating dendrite branching order over different treatments, and only treatments develop major orders in contrast with control, How can I compare treatments with control if control dont...

09 October 2018 6,858 3 View

No title

02 March 2021 3,060 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View