Im working with drosophila, and i want to avoid those solvent because the influence the may have in memory.
Hi Daniela María Ramírez ,
Interesting point! PTX is a GABAr antagonist and may produce sudden changes in synchronized burst dynamics in certain neurons, but what was the does or concentration that you used in which you observed issues with memory? and what was that effect on memory?
You may use dimethyl formamide (DMF) with similar concentration that is normally used to prepare and solve PTX in DMSO (approximately 30 mg/ml).
Hey,
How about using just the water? The water solubility for picrotoxin is around 3-4 g/L; 3-4 mg/ml. Also, increasing the temperature of water improves its solubility in water.
Article Characterization of Seizures Induced by Acute and Repeated E...
Is this concentration enough for your experiment?
"Picrotoxin (Sigma-Aldrich, St. Louis, MO) was prepared in distilled water at a concentration of 0.4 mg/ml for intraperitoneal administration and 1 mg/ml for intravenous administration."
There's also the list of others solvents:
- 1 g in 350 ml water (The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983., p. 1069)
- 1 g in 5 ml boiling water (approx, The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983., p. 1069)
- 1 g in 13.5 ml 95% ethanol (The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983., p. 1069)
- 1 g in 3 ml boiling alcohol (approx, The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983., p. 1069)
- Sparingly sol ether, chloroform (The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983., p. 1069)
- Readily sol in strong ammonia water, in aqueous solutions of sodium hydroxide (The Merck Index. 10th ed. Rahway, New Jersey: Merck Co., Inc., 1983., p. 1069)
- Sol in glacial acetic acid and solutions of acids and alkali hydroxides (Reynolds, J.E.F., Prasad, A.B. (eds.) Martindale-The Extra Pharmacopoeia. 28th ed. London: The Pharmaceutical Press,1982., p. 368 )
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