I am facing the problem in culturing H.hepaticus on 5% Trypt Soy Agar plates. I successfully cultured it in the past. Using the same strategy, now I am not able to trouble shoot the problem even after modifying various things. There are the various things to check on like gas mixture, anaerobic chamber, temperature (which usually fluctuates from 37 to 40 degrees) and incubation time. Please suggest.
Sorry, but I haven't experience in culturing with H. hepaticus.
Dear Tabasum,
You may try culturing it on trypticase soy agar with 5 % sheep blood (TSA with 5 % sheep blood; Remel) in an anaerobic chamber under anaerobic conditions (90 % N2, 5 % H2 and 5 % CO2) at 37 °C for 5 days.
I hope the links i attached helps
Regards,
Armstrong
http://mic.sgmjournals.org/content/153/4/1006.long
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC263656/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC228616/pdf/332968.pdf
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