Armstrong Omoregie

5 Questions 16 Answers 0 Followers

Questions related from Armstrong Omoregie

What is the most preferred way of cultivating anaerobic bacteria when in broth media?

I am trying to culture anaerobes, I am familiar with the anaerobic chamber, in the case of growing the microbes on agar media. Can anyone assist me with any suggestions on how to culture these...

23 August 2016 1,968 6 View

Can I get a suggestion on the appropriate voltage and time to run agarose gel electrophoresis of bacterial PCR products?

I have noticed various authors use different voltage and time while running their gel electrophoresis such as 100V for 30 min and 75V for 35 min etc. Can anyone kindly suggest a preferred...

28 September 2015 5,081 6 View

How can you calculate specific urease activity using conductivity meter?

I’m working on screening for locally found ureolytic bacteria strains isolated from soils. I am having challenges understanding a certain part of the protocol which is being used to test urease...

10 January 2015 2,139 1 View

How does one determine OD of culture stock?

I incubated a 10 ml bacteria culture into a universal bottle under shaking condition and had to check the biomass OD600 at every 12 hrs for a duration of 72 hrs. My aim is to record the rate...

11 September 2014 7,596 6 View

How can I determine microbial biomass in relation to Optical Density (OD)?

I am trying to determine a bacterial cell growth curve. I wonder which method will be preferable for me to be able to obtain appropriate/more-reliable data or results from either of the following;...

11 May 2014 3,682 9 View