Does anybody have a strain of MDCK cell line that they know will support replication of A/California/07/09pdm. I will pay any costs. I do have some but I`m getting poor virus titres and have heard the type of MDCK cell can affect growth of different sub-types and strains
Cheers
If you are willing to pay for them, you should just buy them from ATCC. I have purchased MDCKs from them and used them to grow many influenza virus subtypes including the 2009 pH1N1 with no problems.
I know some people have mentioned having trouble growing certain isolates in MDCK cells. You may want to consider another cell type or a modified protocol. Here is another RG discussion on the same topic:
https://www.researchgate.net/post/Do_you_have_any_ideas_to_obtain_high_titers_of_a_pandemic_H1N1_influenza_virus
Thanks Shamus
I've just looked at the ATCC and they are pretty expensive particularly as they
Are being shipped to the UK
is there any chance we could get some from you
In be most grateful
Cheers
Mark
http://www.sigmaaldrich.com/catalog/product/sigma/05071502?lang=en®ion=GB
Hi,
as Patrick Keeler has written, the best way is to get the culture directly from the ATCC. You may also ask in any National Reference Center on Influenza or in the WHO Reference Center (in the UK - NIMR - now rebranded as the Crick Worldwide Influenza Centre). How old is your culture (how many passages did you produce)? Sometimes it's sufficient to recover a new portion of cells from liquid nitrogen to improve virus yield.
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