8 Questions 32 Answers 0 Followers
Questions related from Mark Quinlivan
Hi All If you take a look at the image, the upper virus X assay (for Rotavirus) is giving off a lot of fluorescence at the end of the run (circled) which is clearly not real (we know these are...
03 March 2016 4,085 9 View
can i use human type-o rbcs with h3n2 Texas and Victoria in the HI assay?
08 August 2015 7,742 3 View
Hi guys I'm running the HI assay on 2300 sera using A/California/07/09pdm as the antigen and type0 human rbcs. All sera have been rde treatedbut is itnecessary torrent all by adsorptionwith rbcs...
06 June 2015 9,151 2 View
Hi Guys I`m in need of your opinions on interpretation of HI resuts I`ve attached a couple of annotated plates where I seem to have four different outcomes. Could you read them and say if you...
06 June 2015 588 9 View
I'm having a similar problem. I buy in dmem, the cells look good but the media changed colour to pinkish purple within hours. It looked a bit cloudy but I couldnt see anything unusual under the...
05 May 2015 9,553 10 View
Gi often get nice pellets forming but they sometimes don't seem to run when I tilt the plate, should I count them as antibody positive.for example streaming ofpellets has occurred up until the...
05 May 2015 7,278 3 View
Does anybody have a strain of MDCK cell line that they know will support replication of A/California/07/09pdm. I will pay any costs. I do have some but I`m getting poor virus titres and have...
04 April 2015 2,607 4 View
Guys I`m trying to set up the Influenza HI assay in our lab but I`m struggling to get any virus titre on the HA assay. I grew up H1N1 \California/07 pdm0 in MDCK cells. On day 3-4 good I saw good...
03 March 2015 6,615 13 View
