RBC choice for the HI assay?

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Unwanted Real-time PCR signal - probe degradation - if so why?

Hi All If you take a look at the image, the upper virus X assay (for Rotavirus) is giving off a lot of fluorescence at the end of the run (circled) which is clearly not real (we know these are...

02 March 2016 4,146 9 View

Can anyone help with removal of non-specific agglutinate for influenza haemagglutinaion inhibition assay?

Hi guys I'm running the HI assay on 2300 sera using A/California/07/09pdm as the antigen and type0 human rbcs. All sera have been rde treatedbut is itnecessary torrent all by adsorptionwith rbcs...

05 June 2015 9,207 2 View

Can someone help with the interpretation of Influenza haemagglutination inhibition assay results?

Hi Guys I`m in need of your opinions on interpretation of HI resuts I`ve attached a couple of annotated plates where I seem to have four different outcomes. Could you read them and say if you...

05 June 2015 635 9 View

Can someone help regarding RBC pelletet in the influenza haemagglutination inhibition assay?

Gi often get nice pellets forming but they sometimes don't seem to run when I tilt the plate, should I count them as antibody positive.for example streaming ofpellets has occurred up until the...

04 May 2015 7,318 3 View

Why does my dmem turn purple?

I'm having a similar problem. I buy in dmem, the cells look good but the media changed colour to pinkish purple within hours. It looked a bit cloudy but I couldnt see anything unusual under the...

04 May 2015 9,628 10 View

Does anybody have a strain of MDCK cell line that they know will support replication of A/California/07/09pdm?

Does anybody have a strain of MDCK cell line that they know will support replication of A/California/07/09pdm. I will pay any costs. I do have some but I`m getting poor virus titres and have...

03 April 2015 2,642 4 View

Is there an optimum time point (day/hrs) to harvest influenza infected culture supernatant to obtain a high titre for the haemagluttination assay?

Guys I`m trying to set up the Influenza HI assay in our lab but I`m struggling to get any virus titre on the HA assay. I grew up H1N1 \California/07 pdm0 in MDCK cells. On day 3-4 good I saw good...

02 March 2015 6,677 13 View

Does anyone know why this article is republished using the fake address. Is it about stealing my patent?

This article is mine. It was republished by unknown person using an address (Wilson N Jones Regional Center, Sherman Texas USA. I have a patent.

10 August 2024 7,528 5 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View

Ching-Hui Tai

Yes you can.

I followed the protocol from WHO collaborating lab, VIDRL, and they used human O-type RBC with both Texas (2014) and Victoria (2013) strains.

Sorry I didn't find the online manual of kit 2014. Attached files are manuals for kit 2013 and 2015.

Mark Quinlivan

Great, many thanks for your help