Does anybody have a protocol for quantification of esterase and cutinase activity?

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Shamsher S. Kanwar

A colorimetric assay using p-nitrophenyl acetate as a substrate for assay of esterase may be employed.

Maurice Ekpenyong

As simple as Shamsher has suggested. You could also used p-nitrophenol butyrate (C4) if you do not not acetate (C2). Please see the attached document for details.