Kindly provide a proper protocol for qualitative and quantitative estimation of Esterase enzyme.
A colorimetric assay using p-nitrophenyl acetate as a substrate for assay of esterase may be employed.
As simple as Shamsher has suggested. You could also used p-nitrophenol butyrate (C4) if you do not not acetate (C2). Please see the attached document for details.
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