To calculate the enzyme activity of tryptophan amino transferase. I want to know the molar extinction coefficient. Does any one know the molar extinction coefficient of tryptophan aminotransferase ?
If you know the amino acid sequence of your protein, you can calculate its extinction coefficient when fully denatured in 6M guanidine-HCl using this calculator.
https://web.expasy.org/protparam/
The assumption is that the absorbance is only due to the amino acids, not to any bound ligands or prosthetic groups. The calculation will not necessarily be accurate for a native protein.
Maya Ravunni
Do you mean the enzyme or the product of the enzymatic activity?
Stanley, J.C., Nicholas, A.R., Dickson, A.J., Thompson, I.M. and Pogson, C.I., 1984. Tryptophan aminotransferase activity in rat liver. Biochemical Journal, 220(1), pp.341-344.
Tryptophan aminotransferase was assayed at30°C by following the formation of a complex between borate and the enol form of indol-3-ylpyruvate at 328nm.
https://core.ac.uk/download/pdf/192395584.pdf
I want know the molar extinction coefficient of the product of enzyme activity
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