I've been trying to dissolve amylose in water for an enzymatic assay but I have failed, I would appreciate any suggestion. Thanks
Amylose doesn't dissolve in water according to my findings. A few different experiments I just read online used either: DMSO and ethanol in the procedures, or by dissolving in aqueous alkanols on low heat. PubMed can be a great help with some of your questions. Good luck!
which type of amylose try to resolve it . you can see the link below which involve an article about water- soluble amylose : Preparation and properties by:A. Sarko, F. J. Germino andB. R. Zeitlin. Journal of Applied Polymer Science :Volume 8, Issue 3, pages 1343–1355,
http://onlinelibrary.wiley.com/doi/10.1002/app.1964.070080324/abstract
Amylose, depending on its molecular weight and due to its helical structure formed by two linar macromolecules is only partly soluble in water. Short heating of suspension of this polysacharide in water at 98 oC leads to formation of colloidal solution of soluble fraction and remining insoluble large molecular weight fraction does not dissolve.
When cooling the colloidal solution of amylose certain portion of dissolved polysaccharide may precipitate. Separation of not dissolved and precipitated fractions via centrifuge gives you colloidal solution of lower molecular weight amylose. The amount of dissolved amylose is easy to determine from the total amount of amylose mixed with water minus amounts of the separated solid insoluble and precipitated residua.
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