To determinefree Melatonin in serum or plasma samples is not so easy as it always found to be bounded to large proteins. Countless methods of the pretreatment of serum/plasma samples are described. But those methods use methanol or dichloromethane to extract melatonin and those are tedious and time consuming. I came to know that some commercial kits use digestive enzyme preferably pepsin for extraction. Does any one know what is the concentration of pepsin to be used or any other chemicals can be used?. I kindly request you to share your ideas or experience. Thank you.
Yes, this operation is possible through the definition of derivated spectra of high orders
If you are interested in the free fraction, you can use a saliva sample. This reflects the concentration of free melatonin in blood. Be careful, however, not to use a sample device that is base on adsorption of saliva onto cotton fibers
Article Measurement of melatonin in body fluids: Standards, protocol...
Article Dim light melatonin onset (DLMO): A tool for the analysis of...
T. Harumi, S. R. Pandi-Perumal., G. R. Martinez., P. Di Mascio and Y. Hayashi (2007) T. Harumi, S. R. Pandi-Perumal., G. R. Martinez., P. Di Mascio and Y. Hayashi (2007) Chromatographic Analyses of Biogenic Melatonin and Its Related Indoleamines. In. Melatonin: From Molecules to Therapy, S.R. Pandi-Perumal and D.P. Cardinali (eds.), Nova Science Publishers, USA.
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Extraction