In the past, M.Moser of ULB had shown that it took less than five minutes to see the DCs of mouse skin migrate to the draining lymph node.

Subsequently G. Gautier, Paris Diderot, shows the very important role of SEM6A, a semaphorin responsible for migration, which is extremely labile.

On the other hand, the conjugate roles of MMP12 to leave the surrounding tissue, and CCL20 and CCL21 chemokines via CCR6 and CCR7 for migration have been confirmed, notably in cancer (S. Lebecques, CRCL).

All of these are very complicated to get with MoDCs, and in vitro, involve special activations (CD154) and the action of additional cytokines (IL4, IL10 and IFNg)

Hi Lisa

Check out this article, which gave mature DC a rate of 2-3 um/min-1 in the node.

Hope that helps

Bruce 

Thank you both very much! You helped me a great deal , I will do my timelapse next week