Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Sequence of an unknown Oligonucleotide ?

Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .

04 February 2021 5,614 3 View

How to separate solid NHS-folate during the folic acid activation?

Am functionalizing the metal nanoparticle with folic acid. in the process I have taken folic acid in DMSO and added DCC and NHS to activate the carboxylic acid group of folic acid in the standard...

01 February 2021 9,890 3 View

In online GC-FID/TCD connect to flow reactor, why I am getting instable peak area in FID in every injection under same conditions?

We have online GC-FID and GC-TCD (Mod: 2014) connected with flow reactor system. This GC-FID/TCD is connected to a gas line coming from flow reactor system which I used for cascade reaction. In...

11 November 2020 4,999 6 View

Please suggest that the way of obtaining the concentration (in mol) from the gas mixture (N2=98%, Ethylene=1% and Butadiene=1%) is correct or not?

I have a gas mixture (N2=98%, Ethylene=1% and Butadiene=1%). I want to obtained the molar concentration of each components for 1mL volume of the gas mixture. For that purpose, I have used the...

29 September 2020 6,233 1 View

What should be the minimum and maximum dopant concentration to achieve best photocatalytic activity of any semiconductor?

I am working on modifying the band structure of TiO2 semiconductor for visible light photocatalytic activity. For this reason, I would like to dope the metal and/or nonmetal ions into the TiO2 to...

23 September 2020 1,063 6 View

How to calibrate a GC-FID where two of the components are gas phase & others are in liquid phase?

I am new in GC-FID and TCD measurement technique. I will analyze a gas stream at high temperature above 190 oC in the GC-FID system. I want to construct a calibration curve for the mixture of...

08 September 2020 6,073 3 View

Anybody has an experience on the DSSAT 4.7 model ? please let me know?

I am working on the DSSAT model, its a little bit harder than I think please help me?

04 May 2020 5,708 3 View

Why are the COVID-19 infection and mortality rates lower in India?

It is intriguing that the number of reported cases infected or died due to COVID-19 infection in India is comparatively much lower than the United States (US), China and other European countries.

18 April 2020 2,170 22 View

How to convert netcdf file in *.nc4 format to .csv or excel format containing data on planetary boundary layer height?

Monthly average data on Planetary boundary layer height is downloaded from disc.gsfc.nasa.gov. It is in netcdf file in *.nc4 format. How to convert this into .csv or excel format ?

15 December 2019 8,137 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View