Does any of you have protocol for insect cell (Sf9) expression protocol?

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Are you interested in contributing a book chapter on biomaterials in Orthopaedics?

I am editing a book for Springer Nature on recent advancements and future trends in biomaterials for orthopedic and trauma applications, with a publication date slated for early next year. If you...

29 July 2024 5,015 9 View

Are all indexing bodies for the journal's evaluation similar?

A Guide to Journal Indexing Academic journals undergo evaluation by various indexing bodies to ensure ethical practices and high publishing standards. Three prominent indexes include: · PubMed...

27 June 2024 4,415 0 View

What are applications of Class 1, 2, 3 and 4 steel s/c? Can Class 4 s/c considered as flexural members? Why are Class 4 sections not adopted as beams?

Where are Class 1, 2, 3 and 4 sections generally adopted by steel construction industy? Why are class 4 sections not used as beams? As what structural members are Class 4 members used?

10 June 2024 2,453 1 View

Do you know that the Scopus has released the Cite Score of 2023 today?

Please check these related to your journals at: https://www.scopus.com/sources.uri?zone=TopNavBar&origin=searchbasic The Journal Impact Factor will be released by Clarivate in about two...

04 June 2024 1,281 1 View

How can I calculate the persistence length of a polymer using the gmx polystat command?

Hello everyone. I want to calculate the persistence length of a polymer using the gmx polystat command. It gives the value of the radius of gyration and end-to-end distance in nm. However, for...

04 April 2024 5,346 0 View

Neutralization buffer alternate ?

Typical neutralization buffer used for Protein A/G affinity separations is 1 M Tris/HCl pH 9.0 Any alternate buffer for 1 M Tris/HCl ?

04 March 2024 518 2 View

Is it possible to develop an ML algorithm for NTC, that works at synchronization of line speed (below 30 ms) using FPGA?

Is it possible to develop an ML algorithm for NTC, that works at the synchronization of line speed (below 30 ms) using an FPGA board? Like a next-generation firewall.

26 February 2024 6,254 0 View

Suggest me any good journal papers for the efficient NTC on real time traffic using FPGA and ML algorithms?

I am looking for a few good journal papers for my next work, which is used for efficient network traffic classification (NTC) using FPGA and ML algorithms for real-time NTC. It is a kind of...

26 February 2024 4,037 2 View

Do you know that the hand grip strength is a valuable bio health marker?

The HGS can be measured reliably with a hand-held dynamometer. The cut-off values are different in various populations. It is lower in Asians, women, less educated and privileged, and those...

09 January 2024 4,886 4 View

How can we improve the wettability of Carbon fiber with YSZ slurry?

As there is a significant difference in their CTE, can we make composite out of carbon fiber and YSZ matrix through slurry impregnation..? Even though am desizing the carbon fiber at 550C and...

19 October 2023 330 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View