Raju Tippana

6 Questions 10 Answers 0 Followers

Questions related from Raju Tippana

Protein purification from Sf9 insect cells

Hi, I am trying to purify the chimeric fusion proteins containing NGF and various functional domains of Fibronectin. Although lower molecular weight proteins were secreted into the conditioned...

22 February 2017 6,998 5 View

NGF Characterization assays on PC12 Cells?

can any one suggest what potential experiments could be performed to test the NGF activity on PC12 cells except Neurite outgrowth, migration & proliferation.

24 April 2016 2,240 3 View

What kind of characterization assays can I perform to deal with the function of chimeric fusion protein on peripheral nerve regeneration?

I'm planning to perform Neurite outgrowth, migration and proliferation assays on ATCC's Schwann cells and PC12 cells to characterize the function of novel chimeric fusion protein developed by me....

30 April 2015 8,032 2 View

What is the best method for purification of His-tagged recombinant human nerve growth factor?

I tried with Ni-NTA Agarose (Qiagen) using native conditions. I also did Q-Sepharose (ion exchange), followed by Ni-NTA native conditions. Purity was not achieved in either case. Now I'm trying in...

17 October 2014 7,016 2 View

What are the best cell based migration assays for peripheral nerve regeneration?

I'm doing my PhD on development of a novel tissue targeted Growth Factor: ECM chimeric fusion protein as a potential therapy for peripheral nerve regeneration. Part of this study is that I have...

21 June 2014 3,845 0 View

Does any of you have protocol for insect cell (Sf9) expression protocol?

I'm doing my phd on peripheral nerve regeneration. My vector pib/v5-his.

15 January 2014 1,005 2 View