We are looking for applying this technology in Klebsiella pneumoniae cells. Do any of you know any company who offer this kind of service for bacterial cells?. After searching in the internet, I only found the service available for eukaryotic cells.
You might want to take a look at the addgene website.
https://www.addgene.org/CRISPR/
Recently I've met researchers from India done it on E.coli and Bacilli, they're from Indian Institute of Technology (IIT) Assam, Department of Biotechnology
Hi, Andres. I am also ready to apply CRISPR/Cas9 in Klebsiella pneumoniae. Whether your plasmids worked well ? If so, which one you used？
THANK YOU.
In case you're still looking, a paper just came out showing CRISPRi (gene repression) in Klebsiella pneumoniae. No knockout, but could be helpful: Article Engineering CRISPR interference system in Klebsiella pneumon...
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