Hi, I would like to mark the tip location of a NeurNexus probe. I know a good way to easily find the electrode location is to anesthetize the animal, perform an electrolytic lesion, wait a few days for a scar to form, then perfuse the animal and locate the scar. I found this (https://www.researchgate.net/post/How_can_I_mark_the_point_at_which_I_am_recording_in_the_brain_to_see_where_I_was_recording_from) ResearchGate question thread which in turn pointed to several articles suggesting a protocol in which a DC current of approximately 20 to 30 microamps is delivered for approximately 20 to 30 seconds to the site. However, it is not clear to me what device is needed to deliver such small currents: for instance the standard DC current generator in my lab goes down to 0.1 milliamp. An internet search also didn't provide any clear hits for micro-amp range current generators. So my question is: what device do people use for this application?
Moreover, I have also seen others suggesting using substantially higher currents. So, perhaps a somewhat higher current (such as that provided by my counter-top current generator) would also work OK for this task?
Thanks for any insights!
For posterity: I recently learned from some colleagues that the unit people seem to use for this is the World Precision Instrument A365 Battery-driven Stimulus Isolator - this is currently going for around $1700. If anyone knows of a cheaper option (this seems like overkill to me for what in theory should be a fairly straightforward circuit) please do let me know.
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