Subin R. C. K. Rajendran

10 Questions 37 Answers 0 Followers

Questions related from Subin R. C. K. Rajendran

How to get fluorescence scan (tryptophan emission spectrum) peak from the baseline?

For identifying variations in protein folding, tryptophan fluorescence emission scan was performed using microplate reader (samples in black plate). The excitation wavelength was at 290 nm and...

19 November 2015 5,442 12 View

Is anybody working on protein adsorption on nano-emulsion surfaces?

What are the different characteristics that are usually considered for characterizing protein coated nano-emulsions?

04 May 2015 6,703 3 View

Does anyone have an idea about the availability of N-analyzer for protein in the North India?

I want to estimate the N content from the lyophilized protein sample. Can you let me know if this equipment is available for the use?

13 February 2015 9,738 1 View

What are the problems associated with regenerating plants when transformed by Agrobacterium?

Plant regeneration seems to be a problem associated with Agrobacterium mediated transfer because of which people are working towards viral vectors. Can someone elaborate what exatly the problems...

24 October 2014 7,442 4 View

What is meant by context dependent specificity of TALENs and zinc finger nucleases?

Zinc finger nucleases and transcription activator-like effector nucleases are said to suffer from context dependent specificity. Can someone explain what that indicates? How is it a drawback in...

19 October 2014 4,514 2 View

Can splicing occur when a bacterial gene is transfected in an eukaryotic system?

Will splicing occur in a countinuous gene expressed in a eukaryotic system, if so, what steps can one take to prevent such potential problems?

08 October 2014 8,312 3 View

How do I quantify protein extracted from cereal flour with 70% ethanol?

Is there any specific standard method to quantify 70% ethanol soluble protein extracted from cereal flour?

05 October 2014 4,043 5 View

Can lentivirus or adeno-associated virus mediated transfer of RNA or DNA be made in plant cell?

Can viral gene delivery methods be employed in plants? Is there an alternate way to introduce RNA or DNA in plants for genome engineering by CRISPR-Cas system. If a model plant where to be made...

04 October 2014 6,397 6 View

After synthesizing nanoparticles from an emulsion using a surfactant with a sonicator, How should one proceed further?

Protein based nanoparticles are synthesized from an emulsion of oil and aqueous solution by providing energy from a sonicator and a surfactant is used sometimes for stability. Can the solutions...

02 October 2014 1,108 8 View

How can I separate out large quantities (2-5 grams) of seed storage proteins from the milled flour of cereals?

My research involves the seed storage protein alpha prolamin which is alcohol soluble. Is there any method by which I can extract larger quantities of this protein from the milled flour of cereal...

20 September 2014 8,168 2 View