No smears on gel after cDNA synthesis ?

Hi all, I have a problem with detection of cDNA synthesis where when I put it on the gel , there were any smears so what the meaning of that and what can I do? Thanks

21 December 2020 1,126 2 View

Is e.coli ok at Electroporation at 30C?

For reference: I am transforming a newly assembled plasmid what happened: I accidentally incubated my electroporated e.coli cells on the shaker at 30C for 1 hr rather than 37C and did not...

02 December 2020 7,133 5 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

How to develop a framework/model and present it in an analytical form?

Dear All, I am conducting a study on DBD actuators and I'm trying to develop a framework for actuators using plasma-based metamaterials. In order to reach this objective, I need to learn how to...

01 June 2020 3,864 2 View

Duplex PCR Target A & IC & target B & Ic works great. When multiplex the three together A FAM , B HEX, IC Cy5 , A & IC fine, B curve plateau strange?

The curve by B in Hex starts great but the plateau looks like zigzag. I tested different primer concentrations and different annealing temperatures. We use Lightcycler 480 II. Color compensation...

10 May 2020 7,879 3 View

Are ACEIs underdeveloped in children?

COVID19 shows less affection of lung in children . this may be due to underdeveloped or decreased expression of ACEI in lungs of children taking in consideration that as age increase from 5...

28 March 2020 2,448 2 View

Solution not converged at time ........... in ansys?

I am doing four point flexural text on simply supported beam using ANSYS APDL first with steel plate for loading and supporting and then without it. In both cases there is a problem of non...

19 March 2020 4,775 6 View

Can we find the Link between Teaching Methods and Achievement in Math in Computer-Assisted Elementary Schools?

I published a paper in: Creative Education, Published Online http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47205 The Link between Teaching Methods and Achievement in Math in...

14 February 2020 1,408 4 View

How to detrend a series in Stata when it has a stochastic trend?

08 February 2020 1,803 3 View

What is the editorial office email of this journal?

I submitted an article and received an email stating that I need to fix the word count to 5000 or less., though the word count in my manuscript is 4330. I sent back an email in this regard and...

06 October 2019 9,154 3 View

No title

02 March 2021 3,060 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View