I also need to know how to fix them, my co-labs recommended glutaraldehyde+ ethanol, I saw many protocols containing formaldehyde.
I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.
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Hi there, this is Dina, a postgraduate student at Warwick University. I am working on my dissertation regarding AI and business. I would like to ask some questions for researchers in this field....
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I need the particle size and the shape of graphene oxide in epoxy powder as shown in the following FESEM morphology
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Hello everyone, I'm currently developing HCC organoid and I'm having a difficult time in Qrt PCR because of the house keeping genes. I tried GAPDH but it gets affected in HCC and Beta-actin where...
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I am currently working with agarose and I want to add it to cells but it keeps solidifying so quick before I get to seed them. What is the appropriate percentage of agarose should I use? and If it...
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Hey everyone, I’m relatively new to the field of mass spectrometry and proteomics and need some advice. I’m trying to run a recombinant protein on our mass spec. It should be a really...
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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
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Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
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Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
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I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
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