I am interested in determining if there is a correlation between stimuli that affects an individual's feelings and (incline/decline) effect towards one's cell development--neurons.
I know anxiety and stress has a direct effect on the mitochondria of the cell and impact functioning. There are many good articles on this topic.
I'm starting my PhD in Fisheries and Aquaculture Bioeconomics. My thesis is focused on analyzing bioeconomics related to the gillnet multi-specific fishery of elasmobranchs in Peru. I would ask...
25 September 2022 4,555 3 View
Hello everbody. I´m working with IQF fluorescence assays from Lifesensors Company. But I´m having problems when I try to reproduce the data by duplicate and triplicate. I would like to know if...
16 September 2021 9,583 1 View
I have tried with chloroform-methanol (2:1 v/v) but the emulsion produced after sonication is not stable. Also, lipids don't dissolve well in pure ethyl ether.
24 February 2020 2,592 5 View
Hello, I would appreciate any reference that help me find out the enthalpy value of glycosidic bond breakage between N-acetyl-glucosamines (or chito-oligosaccharides) Thanks in advance!
20 August 2019 1,004 1 View
Hi, my problem is that i'm using actin as a housekeeping/reference gene for my real time PCR assays and its Ct are not stable as i would wish they were. So i started using another gene, EIF4, as a...
27 June 2017 570 0 View
My problem is that i'm trying to precipitate RNA with a Ethanol RNA Precipitation protocol and the yield is bad (almost half of initial concentration and half 260/280 purity). My initial volume is...
30 May 2017 3,847 3 View
Hello everyone, I'm currently working on my research using DAPC for analyzing the genetic structure of my quinoa population using SSR. Before doing the DAPC, there is a prior step where I use the...
18 January 2017 811 6 View
Argonauts were found in a stomach of a shark.
14 November 2016 4,361 2 View
Found on blue shark,
14 October 2016 3,103 5 View
I am
05 April 2016 9,810 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
I'm an undergraduate university student conducting research on the effects of internet use on depression and anxiety among undergraduate Ghanaian university students: Mediation role of Internet...
31 July 2024 2,979 3 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
18 July 2024 2,420 0 View
there can be some triggering situations, how do you handle them?
18 July 2024 7,076 3 View
To us men, sex is sex. We want it, let’s do it, we’re done. For many women it isn’t always that simple. You need to be in tune with her emotionally if you want to make her more receptive sexually....
15 July 2024 4,495 1 View
To be fair women are also guilty because she does not feel confident or comfortable to say to her partner, 'Hey, sex needs to be about what I want. And tonight it’s going to be an...
14 July 2024 5,936 11 View
Unlike humans, it is believed that birds do not have a symbolic language system that can be reduced to words (Berwick et al. 2012). But they do have a nervous system that can generate sequences...
14 July 2024 9,852 0 View
I came across the Green Building and Sustainable Architecture collection under Nature Scientific Reports some weeks ago. https://www.nature.com/collections/gajghaebce The special issue/collection...
10 July 2024 5,533 1 View