What solvent mixture/ratio should I use for preparing liposomes by REV method?

I have tried with chloroform-methanol (2:1 v/v) but the emulsion produced after sonication is not stable. Also, lipids don't dissolve well in pure ethyl ether.

24 February 2020 2,360 5 View

What is the minimum volume for RNA precipitation ?

My problem is that i'm trying to precipitate RNA with a Ethanol RNA Precipitation protocol and the yield is bad (almost half of initial concentration and half 260/280 purity). My initial volume is...

30 May 2017 3,726 3 View

What happens in this electrochemical cell?

I am

05 April 2016 9,691 3 View

How to make refractory bricks?

Another project of mine is manufacturing fire bricks, but I can't find a composition to begin with. Would you help me with this?

03 February 2016 1,728 1 View

How to design a single-screw extruder?

I'm working on designing a pasta machine, but I can't find the equations for designing the screw of the extruder. Would you help me with this info?

03 February 2016 488 2 View

How do I simulate a solar collector in ANSYS fluent?

I would like to ask you for a reference for simulating one tube of a solar collector in ANSYS. I will attach a model of the tube I'm trying to simulate. In fact I would like to do what is depicted...

07 April 2015 4,334 9 View

Is this a Triakis maculata specimen?

30 November 2014 8,829 6 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is it Ok to decline to write a chapter for a IntechOpen to the lack of experience?

I have been asked by IntechOpen to write the chapter for a book they are going to publish, but as I don't have enough experience in the area they proposed me to write I declined their offer. Now...

24 February 2021 2,802 4 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

Is it appropriate to use AIDA model (Assessment of Identity Development in Adolescence) on a non-clinical sample?

I am researching emotion regulation and identity development in adolescence. I've come across several inventories for assessing identity development and it seems like the AIDA scale is the most...

16 February 2021 1,261 1 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View