Has anyone had issues staining AAV injected tissue with immunofluorescence?

I am injecting the hippocampus with an AAV8-GLT1 viral vector. I noticed when I am staining with immunofluorescence the secondary antibodies (Ms 598) is sticking to AAV virally injected tissue...

19 March 2020 5,961 5 View

What solvent mixture/ratio should I use for preparing liposomes by REV method?

I have tried with chloroform-methanol (2:1 v/v) but the emulsion produced after sonication is not stable. Also, lipids don't dissolve well in pure ethyl ether.

24 February 2020 2,360 5 View

Does anxiety create a direct effect on the person's feelings alone or is there an effect to one's cell development?

I am interested in determining if there is a correlation between stimuli that affects an individual's feelings and (incline/decline) effect towards one's cell development--neurons.

20 October 2019 9,760 1 View

Can anybody help me with the protocol on how to detect protease activity in a gel containing Casein substrate?

I have just cloned and got a soluble expression(50%) of a thermophilic serine protease and did the heat treatment for purification at 70 and 80 degree with over 90% purity, and also have a...

15 March 2019 9,330 3 View

What are best purification buffers and their conc. that I can use to purify a 6xHis- tagged recombinant serine protease using Ni-NTA resin ?

I have just cloned and expressed a thermophilic alkaline serine protease in E.coli BL21(D3). The protease was expressed as a soluble protein (about 60%) with about 30kDa Mol. wt. (confirmed by...

27 December 2018 1,777 7 View

Can i cytospin cells that are already fixed and stained for IF onto glass slides for confocal imaging?

I plan to isolate breast cancer cells from patient blood samples and would like to fix and stain them in-situ, within the Eppendorf, and then spin them onto a (poly-l-lysine-coated) glass slide...

14 August 2018 3,354 3 View

Can MOLT-4 cell be stimulated with anti-CD3/anti-CD28?

I am looking for a cell line other than Jurkat cells that can signal through the TCR. Would this be possible with MOLT-4 cells or do you know of other cells lines that I could use?

07 June 2018 651 3 View

I'm running a comeptitive ELISA and when i add my 0.15M HF stop solution (with 0.1MEDTA) the net OD decreases and it should increase. help!?

I'm running a comeptitive ELISA and when i add my 0.15M HF stop solution (with 0.1MEDTA) the net OD decreases and it should increase. help!?

31 May 2018 9,776 2 View

What is best temperature for the incubation of thermophilic bacteria on liquid broth for the purpose of DNA extraction?

I incubated my organism (thermophillic bacteria) on LB broth at 37 and 40 degrees centigrade for the purpose of extracting DNA from the organism but did not show any growth after 22hrs of...

05 October 2017 601 11 View

Are there any publically available DNA database for recA gene sequences to use in metagenomic analysis?

14 August 2017 1,969 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View